| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.158185 |
| Chromosome: | chromosome 7 |
| Location: | 1208209 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g321150 | CYG15 | Adenylate/guanylate cyclase, nitric oxide sensing; (1 of 5) K12319 - guanylate cyclase soluble subunit beta (GUCY1B) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGAGGGAAGGCCAGCTGTCCGTGCCTGTG |
| Internal bar code: | GGACGACAGGGGCAGGGGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1150 |
| LEAP-Seq percent confirming: | 98.9202 |
| LEAP-Seq n confirming: | 3481 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCAGAAGCGAGTAAACCAG |
| Suggested primer 2: | CGTGCCACTTATCCAAACCT |