Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.158194 |
Chromosome: | chromosome 8 |
Location: | 633177 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre08.g359900 | (1 of 19) K08850 - aurora kinase, other [EC:2.7.11.1] (AURKX) | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTTCTCGGGCCGCAGCTTCACGCGTGGTT |
Internal bar code: | ATCTTCGTCTTCGTTTTTGTGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 829 |
LEAP-Seq percent confirming: | 98.4523 |
LEAP-Seq n confirming: | 2290 |
LEAP-Seq n nonconfirming: | 36 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATCGTAACAAGGTCAACGCC |
Suggested primer 2: | TGAGCTCGTGATGGAGTGAG |