| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.158199 |
| Chromosome: | chromosome 16 |
| Location: | 5379953 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g681550 | CSV13 | Chlamydomonas-specific family protein | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGCAATCCCAGCGTTCTCAACAGCGTCC |
| Internal bar code: | ATAGGCGATCCTCTTGCGGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 524 |
| LEAP-Seq percent confirming: | 99.274 |
| LEAP-Seq n confirming: | 1094 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTAGCGCAGCTACTCTCG |
| Suggested primer 2: | ACCTCACTACCGTACGTGGC |