| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.158199 |
| Chromosome: | chromosome 16 |
| Location: | 5379978 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g681550 | CSV13 | Chlamydomonas-specific family protein | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAAGTCATTGAACTCTTCGCTTAAGGTTC |
| Internal bar code: | CGGATCGCATTCAAACCCGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 987 |
| LEAP-Seq percent confirming: | 98.9799 |
| LEAP-Seq n confirming: | 3105 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGTGTGGCTTGAGAATGC |
| Suggested primer 2: | ACCTCACTACCGTACGTGGC |