Insertion junction: LMJ.RY0402.158219_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.158219
Chromosome:chromosome 10
Location:5468310
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre10.g458850 SRR32 Scavenger receptor cysteine rich (SRCR) protein; (1 of 20) IPR001190//IPR017448 - SRCR domain // SRCR-like domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCAGACGGCACGCTACCGTACTCGCACCAG
Internal bar code:TGCGTCGGCGAGGCTCGAAAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:699
LEAP-Seq percent confirming:70.9266
LEAP-Seq n confirming:4118
LEAP-Seq n nonconfirming:1688
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:TCAATAAACTCAGGGGGTGC
Suggested primer 2:CGCCCTTTTTGTGGCTATTA