| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.158228 |
| Chromosome: | chromosome 16 |
| Location: | 4378119 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g671400 | ARS1 | (1 of 2) IPR000917//IPR012083//IPR017850 - Sulfatase // Arylsulfatase // Alkaline-phosphatase-like, core domain; Periplasmic arylsulfatase 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGACTTGATCTGAGCAACGCCCTTGTCGC |
| Internal bar code: | GGTGGCTAAGAGTGCGCGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 466 |
| LEAP-Seq percent confirming: | 99.3029 |
| LEAP-Seq n confirming: | 3704 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCCCACTATTCCTGTCGT |
| Suggested primer 2: | TCCTGATGCCCGTTCTTATC |