| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.158261 |
| Chromosome: | chromosome 1 |
| Location: | 5758207 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g041100 | HLM3 | (1 of 1) PTHR12197:SF75 - HISTONE-LYSINE N-METHYLTRANSFERASE ATXR2; Histone-lysine N-methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAATCAGCTCGTAATCGGCCTGACACCCC |
| Internal bar code: | CCCTCGGGCCTAGCGGGCAATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 724 |
| LEAP-Seq percent confirming: | 99.0148 |
| LEAP-Seq n confirming: | 603 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGACCGTCTGACCACAGAAT |
| Suggested primer 2: | TACACCGCTCGCACTTACAC |