Insertion junction: LMJ.RY0402.158270_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.158270
Chromosome:chromosome 17
Location:2556766
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g716600 (1 of 752) IPR027417 - P-loop containing nucleoside triphosphate hydrolase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGATTTGACGGCGGGAAGGGGGGGTGTTGG
Internal bar code:CGCCTACCGACGATCCGCTGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:812
LEAP-Seq percent confirming:99.76
LEAP-Seq n confirming:1663
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:AGAGAAGGAAACACGCAGGA
Suggested primer 2:GGGCTTGTTCCATTTCGTTA