| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.158278 |
| Chromosome: | chromosome 16 |
| Location: | 1677905 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g654600 | PHC56 | (1 of 71) PF12499 - Pherophorin (DUF3707); Pherophorin-chlamydomonas homolog 56 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATGTTGCCAAAACTTTACCGCTTCACAAC |
| Internal bar code: | AGGTAGTCTACTGCTTGACACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 434 |
| LEAP-Seq percent confirming: | 85.6698 |
| LEAP-Seq n confirming: | 550 |
| LEAP-Seq n nonconfirming: | 92 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTAGCCAGATAGAGCCGTG |
| Suggested primer 2: | AACGACGGCACCTTTTACAC |