| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.158280 |
| Chromosome: | chromosome 2 |
| Location: | 1683486 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g085850 | ARS6 | Arylsulfatase; (1 of 19) 3.1.6.1 - Arylsulfatase / Sulfatase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGACTGGGGACGTTGTCCGGCAAAGATTA |
| Internal bar code: | GAATCCGTGTGTAGCGTCAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 796 |
| LEAP-Seq percent confirming: | 97.8242 |
| LEAP-Seq n confirming: | 2293 |
| LEAP-Seq n nonconfirming: | 51 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGCCCAAACTAAACCAAA |
| Suggested primer 2: | TCGATAGACTGGACGCACTG |