Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.158292 |
Chromosome: | chromosome_1 |
Location: | 4451310 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre01.g030200 | PP2C4,PPP3 | Phosphoprotein phosphatase 2C delta | antisense | 5'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CTAGATAAGAAAGCTTGAACGAATGGGCTG |
Internal bar code: | ACCTGCCAATACCCGCTGACCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 988 |
LEAP-Seq percent confirming: | 99.4368 |
LEAP-Seq n confirming: | 3708 |
LEAP-Seq n nonconfirming: | 21 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCAATTGTCCTGGTTGGTCT |
Suggested primer 2: | CTCTTGCACCCCATCGTAAT |