| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.158307 |
| Chromosome: | chromosome 12 |
| Location: | 8634310 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g546900 | (1 of 24) IPR006553 - Leucine-rich repeat, cysteine-containing subtype | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTGCACCCGCCCAAGTCCACCACACGCA |
| Internal bar code: | TCGGATGGGATTGGACAAGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 355 |
| LEAP-Seq percent confirming: | 79.8727 |
| LEAP-Seq n confirming: | 1631 |
| LEAP-Seq n nonconfirming: | 411 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGATGCTATGGGGTCCTGA |
| Suggested primer 2: | CCCAACATGTCACACACACA |