| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.158331 |
| Chromosome: | chromosome 6 |
| Location: | 1928065 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g263602 | PEX12 | Peroxin 12; (1 of 1) K13345 - peroxin-12 (PEX12, PAF3) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGACTGGCTGTGGGGAAGGGTGGGGGGGG |
| Internal bar code: | GGTTGATCCCGGTTTAGGGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1090 |
| LEAP-Seq percent confirming: | 99.6599 |
| LEAP-Seq n confirming: | 1465 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCTCCACGCATTCTGAAC |
| Suggested primer 2: | ACCTGCCCATTACCCTATCC |