| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.158341 |
| Chromosome: | chromosome 16 |
| Location: | 6347745 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g674100 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCCCGAGGCGGCGCAGGAGTGCAGGACCT |
| Internal bar code: | CCCCCGGTTTAGTCGATAAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 629 |
| LEAP-Seq percent confirming: | 98.1942 |
| LEAP-Seq n confirming: | 2012 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGAGCCTGTGTCCTTGCCT |
| Suggested primer 2: | TCGAATCCTCGTGTCCCTAC |