| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.158391 |
| Chromosome: | chromosome 6 |
| Location: | 3763871 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278157 | (1 of 1) 3.2.1.143 - Poly(ADP-ribose) glycohydrolase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGGCCATCGTAGGTGAGTGGCGGCGTCA |
| Internal bar code: | GCTTTTTTGTGGACTTTGGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 183 |
| LEAP-Seq percent confirming: | 99.3756 |
| LEAP-Seq n confirming: | 3024 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGCCATGTACAGGATGC |
| Suggested primer 2: | ACCTTATCATCCGTGGCTTG |