| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.158426 |
| Chromosome: | chromosome 10 |
| Location: | 3902290 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g447850 | YEE2 | (1 of 3) K07112 - uncharacterized protein (K07112); Integral membrane protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATGTCATGTTGAACAAAGCCATACGGTAC |
| Internal bar code: | AGGTCATGTGAAGGCTAGCTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 432 |
| LEAP-Seq percent confirming: | 96.5745 |
| LEAP-Seq n confirming: | 70144 |
| LEAP-Seq n nonconfirming: | 2488 |
| LEAP-Seq n unique pos: | 139 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCCCTGTACTCTGCTCACC |
| Suggested primer 2: | CGGTGTGTGTGTGTGTGTGT |