| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.158446 |
| Chromosome: | chromosome 3 |
| Location: | 7626930 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g204400 | (1 of 1) K09651 - rhomboid domain-containing protein 1 [EC:3.4.21.-] (RHBDD1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACCGCCGGAGCTGTTGCCGTACATGAACG |
| Internal bar code: | AAGTCACGCCAGTACAATGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 364 |
| LEAP-Seq percent confirming: | 90.1818 |
| LEAP-Seq n confirming: | 3224 |
| LEAP-Seq n nonconfirming: | 351 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGTCAAGCACTATGGCAA |
| Suggested primer 2: | ACACAGGGTCACATCGCATA |