| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.158458 |
| Chromosome: | chromosome 12 |
| Location: | 7731862 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g553900 | CNG2 | (1 of 1) K04905 - potassium voltage-gated channel Eag-related subfamily H member 2 (KCNH2); Cyclic-nucleotide gated potassium channel | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGTTCGTCCCGATCTCGGCCTCTCGTGGC |
| Internal bar code: | CGTCTCATCGGCAGTGGAAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1130 |
| LEAP-Seq percent confirming: | 99.1191 |
| LEAP-Seq n confirming: | 6976 |
| LEAP-Seq n nonconfirming: | 62 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCACGGCAAACTACATGG |
| Suggested primer 2: | AAGGTACCACCGATCAGCAC |