| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.158459 |
| Chromosome: | chromosome 6 |
| Location: | 6027044 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g289350 | PRF2,PRFA2 | (1 of 2) PTHR11075:SF9 - PEPTIDE CHAIN RELEASE FACTOR 1, MITOCHONDRIAL-RELATED; Prokaryotic-type peptide release factor A | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGACATGTAAGTTCTATTGCCGCGTGCAT |
| Internal bar code: | AGTGGAGCGGGTGCGTTGGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 370 |
| LEAP-Seq percent confirming: | 99.8863 |
| LEAP-Seq n confirming: | 1757 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCCCAAACTATGACTTGA |
| Suggested primer 2: | GACGTGGAGCCTCAAGAAAG |