Insertion junction: LMJ.RY0402.158480_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.158480
Chromosome:chromosome 5
Location:797349
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre05.g248200 (1 of 2) PTHR21493//PTHR21493:SF124 - CGI-141-RELATED/LIPASE CONTAINING PROTEIN // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTTCACCGCCACAAGACCACATTTGCAGC
Internal bar code:TATACAGCACGATGCGTGATGC

Confirmation - LEAP-Seq

LEAP-Seq distance:250
LEAP-Seq percent confirming:53.4183
LEAP-Seq n confirming:1383
LEAP-Seq n nonconfirming:1206
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GCGACGGACTTTGACTTCTC
Suggested primer 2:GTGTGCGTTGTTGTTTTTGG