Insertion junction: LMJ.RY0402.158490_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.158490
Chromosome:chromosome 16
Location:3026397
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g664950 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGGGCGTCTGCGACGTCGCACTTCGGTCC
Internal bar code:GGGAGGATCCAGGGTGCATCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:139
LEAP-Seq percent confirming:98.4252
LEAP-Seq n confirming:125
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CGTCACCAAACAAGTCCAGA
Suggested primer 2:ATTGAGGCTAACAACCCACG