Insertion junction: LMJ.RY0402.158503_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.158503
Chromosome:chromosome 17
Location:541668
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g699600 SBP2,SEBP2 (1 of 2) 3.1.3.37 - Sedoheptulose-bisphosphatase / Sedoheptulose-1,7-bisphosphatase; putative Sugar bisphosphatase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCAGCTGGCGCCTGCTGCGCAGCGGGGTG
Internal bar code:GCGGGTTATTTGCGGGCGGTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:914
LEAP-Seq percent confirming:98.5785
LEAP-Seq n confirming:1387
LEAP-Seq n nonconfirming:20
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CTACTGAACCCCTCTCTGCG
Suggested primer 2:ACCCACCTCGATCACAAAAG