| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.158537 |
| Chromosome: | chromosome 17 |
| Location: | 4428429 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g731800 | (1 of 2) PTHR10913 - FOLLISTATIN-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGGCAAGACCTACGGCAATGCCTGCGAGG |
| Internal bar code: | TAGCGCAGGCTATGATGGGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 653 |
| LEAP-Seq percent confirming: | 87.4214 |
| LEAP-Seq n confirming: | 973 |
| LEAP-Seq n nonconfirming: | 140 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTTGCAGTGCTTGACCTTG |
| Suggested primer 2: | ATCAAACTCACGTGGGGAAG |