Insertion junction: LMJ.RY0402.158559_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.158559
Chromosome:chromosome 3
Location:4203166
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g174050 MRP4 (1 of 3) PTHR24223//PTHR24223:SF252 - FAMILY NOT NAMED // PROTEIN MRP-2; ABC transporter, multidrug resistance associated protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGACGCGGAAAAGAAGAGAGATTGAGACG
Internal bar code:TAAACAATTTATTGGTATTACC

Confirmation - LEAP-Seq

LEAP-Seq distance:160
LEAP-Seq percent confirming:88.6667
LEAP-Seq n confirming:133
LEAP-Seq n nonconfirming:17
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CGCACGGTAAACTGGTAGGT
Suggested primer 2:GTGTGACACCATTGCTCCAC