Insertion junction: LMJ.RY0402.158568_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.158568
Chromosome:chromosome 3
Location:7653497
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g204100 ELG5 Exostosin-like glycosyltransferase 5; (1 of 34) 2.4.2.41 - Xylogalacturonan beta-1,3-xylosyltransferase / Xylogalacturonan xylosyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTGCACCGCCGCCTTGCTCCCAATGTCCC
Internal bar code:AGTGGAGACTTTGTGTGGTGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1085
LEAP-Seq percent confirming:99.8134
LEAP-Seq n confirming:4815
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:34

Suggested primers for confirmation by PCR

Suggested primer 1:TACAGGTGCGGTTGAGACTG
Suggested primer 2:GGACACATATGCAAACACGC