| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.158574 |
| Chromosome: | chromosome 6 |
| Location: | 3814591 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278167 | (1 of 1) PTHR21037:SF2 - MCG15614 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAACAGGGGTTCCTTCCGACGCCACCTCA |
| Internal bar code: | GGACACGTAACCGGAGAGGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 138 |
| LEAP-Seq percent confirming: | 92.9825 |
| LEAP-Seq n confirming: | 53 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAATGCTATTTGCTGCTCA |
| Suggested primer 2: | AGCGTGAGAGAGACAGAGGC |