Insertion junction: LMJ.RY0402.158592_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.158592
Chromosome:chromosome 10
Location:3361585
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g443950 (1 of 2) PF04564//PF05049 - U-box domain (U-box) // Interferon-inducible GTPase (IIGP) (IIGP) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAATTGCAGGCCCGAACAATACAGAGCGCA
Internal bar code:GCGGCTGTTACCCCGGGTGCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:650
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:210
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTCAGCAGGAAGTAATCC
Suggested primer 2:CGACTAGTCTCCGATGCACA