| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.158593 |
| Chromosome: | chromosome 2 |
| Location: | 5864011 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g111300 | (1 of 5) PF13391 - HNH endonuclease (HNH_2) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTCACGGACGACACGGTCTCGGCCATGG |
| Internal bar code: | AAGGGTGCGCGGTATCGATAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 515 |
| LEAP-Seq percent confirming: | 99.8486 |
| LEAP-Seq n confirming: | 1979 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACAACTACCAATGTGCCG |
| Suggested primer 2: | CCACGTCCAAACCTAGCAAT |