Insertion junction: LMJ.RY0402.158606_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.158606
Chromosome:chromosome 2
Location:3485316
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre02.g095122 FKB16H,FKB16-8,FKB11 Peptidyl-prolyl cis-trans isomerase, FKBP-type; (1 of 25) PTHR10516 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGTACCTCCTGCCCGATTTTGGTGACTTC
Internal bar code:GAGTGGGATGATGGGGGTGACG

Confirmation - LEAP-Seq

LEAP-Seq distance:713
LEAP-Seq percent confirming:90.2041
LEAP-Seq n confirming:442
LEAP-Seq n nonconfirming:48
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:CCCTGTAGCTCCGAGACAAG
Suggested primer 2:GGGCGCATGCATATAGAAAT