| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.158613 |
| Chromosome: | chromosome 6 |
| Location: | 2429236 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g268501 | (1 of 3) PF13563 - 2'-5' RNA ligase superfamily (2_5_RNA_ligase2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCCAATGCTGCAGCATAAAGCCACTTCC |
| Internal bar code: | GACCGAGTACGCTATACGCGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 830 |
| LEAP-Seq percent confirming: | 99.5306 |
| LEAP-Seq n confirming: | 4453 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 54 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCAGCCATATCAGGTCGAG |
| Suggested primer 2: | ATGTGCTCGACGTGACTTTG |