| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.158617 |
| Chromosome: | chromosome 16 |
| Location: | 4022667 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g670300 | PRP39 | (1 of 1) K13217 - pre-mRNA-processing factor 39 (PRPF39, PRP39); Nuclear pre-mRNA splicing factor and U1 snRNP Component | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCAAACTAGCAGCGCTGCGGCTGCAAGG |
| Internal bar code: | GCGCAACGCTGGGTGTATTACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 109 |
| LEAP-Seq percent confirming: | 86.1357 |
| LEAP-Seq n confirming: | 292 |
| LEAP-Seq n nonconfirming: | 47 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCAACAAGTACCTGAGCG |
| Suggested primer 2: | TAACCGTAAGCGGGGTACTG |