| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.158618 |
| Chromosome: | chromosome 10 |
| Location: | 2741700 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g438450 | LTH1 | LAT2 homolog | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGTGGGGAGCTAAAACAGCTCCGCATGGC |
| Internal bar code: | AAGGCAGGGGTGAAAAGAGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 326 |
| LEAP-Seq percent confirming: | 99.5973 |
| LEAP-Seq n confirming: | 742 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGCGACTTGGTCTTGTTG |
| Suggested primer 2: | ACCGGAGACTTCCTCCATTT |