Insertion junction: LMJ.RY0402.158622_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.158622
Chromosome:chromosome 5
Location:1435976
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre05.g244050 Chlamydomonas specific protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTACCGTAATGCTCACGCTGTCAATCACA
Internal bar code:GAGTGTCTCACTCAACGACGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:844
LEAP-Seq percent confirming:99.8171
LEAP-Seq n confirming:3820
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:GTGAGTTTGCCACATCCCTT
Suggested primer 2:ATGATGTGGGGTACGGGTTA