| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.158632 |
| Chromosome: | chromosome 9 |
| Location: | 3384967 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g388801 | (1 of 1) IPR001615 - Delta-endotoxin CytB | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCTGGTGGGACAGCTCGCCCGTCAGCTT |
| Internal bar code: | GGTACAACCGGGGACGCACGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 137 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 198 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTCGTGTGTCGCTCTGAA |
| Suggested primer 2: | AACCACATAGCGGCATTAGG |