Insertion junction: LMJ.RY0402.158639_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.158639
Chromosome:chromosome 17
Location:1080746
Confidence (%):73
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGGCACCCATGCAAGCAACAATTGCCGAC
Internal bar code:TGGTGACGCCCACGAATGAAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1012
LEAP-Seq percent confirming:98.2456
LEAP-Seq n confirming:2016
LEAP-Seq n nonconfirming:36
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:CCAGCTCCTCGTCTATTTGC
Suggested primer 2:CACCATCTTGCAACGGTATG