| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.158666 |
| Chromosome: | chromosome 4 |
| Location: | 468002 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217927 | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCACACTCATCACCTGATCACCTACCGCGA |
| Internal bar code: | GGGGAAACAACAGCGACCGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 837 |
| LEAP-Seq percent confirming: | 99.2323 |
| LEAP-Seq n confirming: | 2973 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCATCGACACTTCAAAAGC |
| Suggested primer 2: | CTAGCCGCCTACTATCCGTG |