Insertion junction: LMJ.RY0402.158713_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.158713
Chromosome:chromosome 17
Location:4333934
Confidence (%):73
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTAAACAGCGACCAGTTATCGGGCCATGCT
Internal bar code:CTGGTTTTGTTCAATCCTAGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:952
LEAP-Seq percent confirming:99.5387
LEAP-Seq n confirming:5826
LEAP-Seq n nonconfirming:27
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:GGCGTGTGATATGTGAGGTG
Suggested primer 2:GACGTAGGATCGGAGTTGGA