| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.158714 |
| Chromosome: | chromosome 9 |
| Location: | 7689319 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g415600 | (1 of 5) 3.2.1.3 - Glucan 1,4-alpha-glucosidase / Lysosomal alpha-glucosidase | 3'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGGTTTGGGGTGGGTTTTGTCTCAATTC |
| Internal bar code: | GAAGTGGTAACCCCCTTTCTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 613 |
| LEAP-Seq percent confirming: | 99.0228 |
| LEAP-Seq n confirming: | 912 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATACGCAGTGTTGTGCTT |
| Suggested primer 2: | GGGCTGTTTGGACACCTTTA |