Insertion junction: LMJ.RY0402.158717_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.158717
Chromosome:chromosome 13
Location:3734385
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre13.g589350 FBB6 Flagellar/basal body protein 6; (1 of 1) IPR000679//IPR002035//IPR013694 - Zinc finger, GATA-type // von Willebrand factor, type A // VIT domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCCCATAGGGGGCCCACTGCGACTGGGCT
Internal bar code:GAGCTGTAAGCCGCGTTTCGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:941
LEAP-Seq percent confirming:99.3769
LEAP-Seq n confirming:638
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GACTGGTGGACGAGGGACTA
Suggested primer 2:CAGTGAGAGGTCCTTGGAGG