| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.158718 |
| Chromosome: | chromosome 5 |
| Location: | 3466619 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g241450 | FTSY1,FTSY | (1 of 1) K03110 - fused signal recognition particle receptor (ftsY); Chloroplast SRP Receptor | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGTGGGGGCGCGAGCCCATGTGCCCATCC |
| Internal bar code: | GCGCACGGGAGAATGCCGGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 905 |
| LEAP-Seq percent confirming: | 99.1297 |
| LEAP-Seq n confirming: | 26653 |
| LEAP-Seq n nonconfirming: | 234 |
| LEAP-Seq n unique pos: | 119 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTACGCCTTCGCAATGTAT |
| Suggested primer 2: | CTCGCACGGTACTACAGCAA |