| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.158719 |
| Chromosome: | chromosome 17 |
| Location: | 2155532 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g712850 | TRX23,TRL9 | (1 of 11) IPR012336//IPR013766 - Thioredoxin-like fold // Thioredoxin domain; Thioredoxin-like protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGTGAGCTCCACAACTGCACCCCCCGCA |
| Internal bar code: | GGCGACTACCGTGATGCGGCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 966 |
| LEAP-Seq percent confirming: | 97.8567 |
| LEAP-Seq n confirming: | 1598 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAGCTACACGGCACGCTAT |
| Suggested primer 2: | GTAGCCAACTCCAAAGCTGC |