| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.158732 |
| Chromosome: | chromosome 6 |
| Location: | 8034827 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g304250 | RLS2 | RegA/Rls-like protein 2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAACGAATTGGTGCAGGACGTTCGAGTTGC |
| Internal bar code: | GTCGGTGTAGCGTTTTTTGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 687 |
| LEAP-Seq percent confirming: | 99.6851 |
| LEAP-Seq n confirming: | 11714 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAAAGCTGTGAGGCGAAAA |
| Suggested primer 2: | GAGGCTCGCCTCATCACTAC |