Insertion junction: LMJ.RY0402.158734_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.158734
Chromosome:chromosome 6
Location:2282068
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g266900 LPA1L Low PSII Accumulation 1 homolog; (1 of 2) PF11998 - Protein of unknown function (DUF3493) (DUF3493) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTCTCGCTGAACGACCTCCGCGGCACGGC
Internal bar code:AAACCAAGAGGCACGCTTGCTA

Confirmation - LEAP-Seq

LEAP-Seq distance:951
LEAP-Seq percent confirming:87.2516
LEAP-Seq n confirming:7597
LEAP-Seq n nonconfirming:1110
LEAP-Seq n unique pos:37

Suggested primers for confirmation by PCR

Suggested primer 1:GGTCTGCTGTCTGATGACGA
Suggested primer 2:GCTGCTCAAACCAGGACTTC