| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.158763 |
| Chromosome: | chromosome 17 |
| Location: | 2226590 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g713500 | HFO22,HFO25 | Histone H4 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTACACCCTGCACACGCTGTATCTGCCACG |
| Internal bar code: | GGATGAGACTAACGGTTGTCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 523 |
| LEAP-Seq percent confirming: | 99.2832 |
| LEAP-Seq n confirming: | 831 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTTGGTGTCCTCGAACAG |
| Suggested primer 2: | ATGAGGGCTGTGTTACCAGG |