Insertion junction: LMJ.RY0402.158850_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.158850
Chromosome:chromosome 2
Location:2720096
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g093600 TEH3 (1 of 6) PF03061 - Thioesterase superfamily (4HBT); Thioesterase-like protein 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGAGCGCAAGAATGCTTTTGAGATGGGAT
Internal bar code:GTCAATGGGCGCCACTGTAGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:277
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:62
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AGAATGCACCCGTAGTCCAC
Suggested primer 2:GAGTGACATGGGCAACACAC