| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.158852 |
| Chromosome: | chromosome 17 |
| Location: | 501385 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g699350 | SMM51 | (1 of 13) PF12847 - Methyltransferase domain (Methyltransf_18); S-adenosyl-L-methionine-dependent methyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTTTCTGGCACACTGTTATGAGGATGTG |
| Internal bar code: | AGCCAAGGAGGCGTAGAAGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 838 |
| LEAP-Seq percent confirming: | 99.8002 |
| LEAP-Seq n confirming: | 999 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATGCGTGTATGTGTGTGA |
| Suggested primer 2: | CGAGGTGGACCGTTAGGTTA |