| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.158861 |
| Chromosome: | chromosome 12 |
| Location: | 5220832 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g528050 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGCCGGACACGGTGCTGGGCGGCGAGGC |
| Internal bar code: | TCACACCGTTTTCATGCGGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1019 |
| LEAP-Seq percent confirming: | 99.5058 |
| LEAP-Seq n confirming: | 30003 |
| LEAP-Seq n nonconfirming: | 149 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATTTAGCAGCCTTTGGAG |
| Suggested primer 2: | ACCTCACGTGTGTTTCCTCC |