| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.158873 |
| Chromosome: | chromosome 9 |
| Location: | 5958519 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g402812 | (1 of 6) PF02656 - Domain of unknown function (DUF202) (DUF202) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCATCTCGCGCCGTGTGTAGCCGCCTGT |
| Internal bar code: | GCCCCCGTCGTCAAACGTGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1051 |
| LEAP-Seq percent confirming: | 98.8511 |
| LEAP-Seq n confirming: | 8776 |
| LEAP-Seq n nonconfirming: | 102 |
| LEAP-Seq n unique pos: | 69 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCAGTTACGACAGGGGTTA |
| Suggested primer 2: | AGCCTGCTCCAACTCCTACA |