| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.158879 |
| Chromosome: | chromosome 14 |
| Location: | 1565024 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g618641 | gene_edge/mRNA_edge/3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGCTTTGCAGCGGCCTCACAGTTGCCGC |
| Internal bar code: | TATACAATACTGTTTTATCCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 824 |
| LEAP-Seq percent confirming: | 73.3027 |
| LEAP-Seq n confirming: | 1274 |
| LEAP-Seq n nonconfirming: | 464 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCGTAATTGCTGTAGGCTG |
| Suggested primer 2: | ACACCACGGCTTCTGGTATC |