Insertion junction: LMJ.RY0402.158880_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.158880
Chromosome:chromosome 12
Location:1818956
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre12.g511950 (1 of 1) IPR003591//IPR013210 - Leucine-rich repeat, typical subtype // Leucine-rich repeat-containing N-terminal, plant-type CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGGGCTGTGACGGCACCCAGCAGCGCGAC
Internal bar code:CGGACACGGCTTGTTTCGAGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:156
LEAP-Seq percent confirming:82.9694
LEAP-Seq n confirming:950
LEAP-Seq n nonconfirming:195
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:AGGAAGGAAAGGGAGGTGAA
Suggested primer 2:AGAAGCCGCAAAGTAGGACA